We are providing this information in an effort to make sure that your bitch gets pregnant.  We want you to have puppies almost as much as you want them. The key to a large healthy litter is breeding at the right time via serum progesterone testing

Paperwork:  Fax  919 932 3575
·        Copy of registration certificate & pedigree
·        Copy of hip certification
·        Copies of all lab tests required, provided by your veterinarian. 

First day of season (or as soon as possible)- Cervical culture and sensitivity. This sample must be obtained using a guarded culture swab such as the Tieglan swab, and from the cervical area, not lower vaginal area.  It takes 4 to 7 days to get final results.  NO bitch will be bred naturally without a C & S, and results must be negative or she must be on appropriate antibiotics for 7 days prior to breeding.  No female with highly resistant or pathogenic bacteria (like Pseudomonas) will be bred naturally (even if she is on the appropriate antibiotics for 7 days). She will be artificially inseminated surgically at her owner’s expense.  

Also get a Canine Brucellosis screening.  Some screenings are positive due to cross reactivity and must have more extensive testing, usually from Cornell.  THIS TAKES TIME.  NO bitch will be bred without a negative Brucella.  

Progesterone timing is required for all bitches bred.  It ensures that the bitch is having a normal heat cycle and has ovulated.  A progesterone series tells us precisely when to inseminate her.  We strongly recommend that you do the majority of the timing tests at your veterinarian, to ensure that you are shipping or driving in with a fertile female. 

Progesterone Timing Chart—Start testing about day 5 to 7 of season.

Below 1.0 ng/ml--no ovarian activity—test every 3 or 4 days
1.0 to 2.0 ng/ml—rise indicates active ovary—test in 2 to 3 days
                2.0 to 4.9 ng/ml—active ovary-test every 24 hours until you determine
    5.0 ng/ml Ovulation
5 to 6 ng/ml—breed in 2 to 3 days
6 to 11 ng/ml—breed in 1 to 2 days
11 to 19 ng/ml – breed today
Over 19—breed immediately if vaginal smear is OK and bitch is still receptive (is considered LATE-hope for the best) 

Progesterone timing in areas where results take days to return are not feasible. Results must be returned within 24 hours (except Sundays) to time the breeding.  The Idexx Veterinary Reference Labs give excellent service using The Idexx Direct Courier Service via FedEx no matter where you are located. Results are faxed and can be accessed online.  Have your vet call 1-800-551-0998 Ext 8327 for info.  You can also use this service for the cultures.  

Bred-Jan   1 2 3 4 5  6  7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27    28 29 30 31
Due--Mar   5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 Apr 1  2  3  4

Bred-Feb   1 2 3 4 5  6  7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26        27 28
Due--Apr   5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30    May 1  2 

Bred-Mar   1 2 3 4 5  6  7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 22 29      30 31
Due--May   3 4 5 6 7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31  Jun  1  2 

Bred-Apr   1 2 3 4 5  6  7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28      29 30
Due--Jun   3 4 5 6 7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30  Jul  1  2

Bred-May   1 2 3 4 5  6  7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29     30 31
Due--Jul   3 4 5 6 7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31  Aug 1  2

Bred-Jun   1 2 3 4 5  6  7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29      30
Due--Aug   3 4 5 6 7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31  Sept 1

Bred-Jul   1 2 3 4 5  6  7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29     30 31
Due--Sept  2 3 4 5 6  7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30  Oct 1  2

Bred-Aug   1 2 3 4 5  6  7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29     30 31 
Due--Oct   3 4 5 6 7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31  Nov 1  2

Bred-Sept  1 2 3 4 5  6  7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28     
Due--Nov   3 4 5 6 7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 Dec       1 2

Bred-Oct   1 2 3 4 5  6  7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29      30 31
Due--Dec   3 4 5 6 7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31  Jan 1  2

Bred-Nov   1 2 3 4 5  6  7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29      30
Due--Jan   3 4 5 6 7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31  Feb  1

Bred-Dec   1 2 3 4 5  6  7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27    28 29 30 31
Due--Feb   2 3 4 5 6  7  8  9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27    Mar 1  2  3  4